Sox2 ELISA Kit for Mouse(Mus musculus) | EIAab.comSox2 ELISA Kit for Mouse(Mus musculus) | EIAab.com

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Sox2 (Gene name),

Transcription factor SOX-2 (Protein name ), SOX2_MOUSE from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Sox2(Sox-2);

Protein name:

Transcription factor SOX-2;

Alternative:

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.

Function:

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

319

Sequence:

50:

MYNMMETELK | PPGPQQASGG | GGGGGNATAA | ATGGNQKNSP | DRVKRPMNAF |

100:

MVWSRGQRRK | MAQENPKMHN | SEISKRLGAE | WKLLSETEKR | PFIDEAKRLR |

150:

ALHMKEHPDY | KYRPRRKTKT | LMKKDKYTLP | GGLLAPGGNS | MASGVGVGAG |

200:

LGGGLNQRMD | SYAHMNGWSN | GSYSMMQEQL | GYPQHPGLNA | HGAAQMQPMH |

250:

RYVVSALQYN | SMTSSQTYMN | GSPTYSMSYS | QQGTPGMALG | SMGSVVKSEA |

300:

SSSPPVVTSS | SHSRAPCQAG | DLRDMISMYL | PGAEVPEPAA | PSRLHMAQHY |

319:

QSGPVPGTAK | YGTLPLSHM

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

String:

UniGene:

SMR:

KEGG:

Uniprot:

FOR

ELISA Kit for Mouse Transcription factor SOX-2

Cat.:

E0423h

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96T

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ELISA Kit for Mouse Transcription factor SOX-2

Cat.:

E0423m

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96T

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ELISA Kit for Mouse Transcription factor SOX-2

Cat.:

E0423c

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MSDS:

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Packing:

96T

CLIA Kit for Mouse Transcription factor SOX-2

Cat.:

U0423c

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MSDS:

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Packing:

96T

CLIA Kit for Mouse Transcription factor SOX-2

Cat.:

U0423m

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MSDS:

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Packing:

96T

CLIA Kit for Mouse Transcription factor SOX-2

Cat.:

U0423h

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Looking for manual.

MSDS:

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96T

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Polyclonal Antibody for Mouse Transcription factor SOX-2

Cat.:

P0423Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse Transcription factor SOX-2

Polyclonal Antibody for Mouse Transcription factor SOX-2

Cat.:

P0423Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse Transcription factor SOX-2

Cat.:

M0423h

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Packing:

40ug

Monoclonal Antibody for Mouse Transcription factor SOX-2

Cat.:

M0423c

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Packing:

40ug

Monoclonal Antibody for Mouse Transcription factor SOX-2

Cat.:

M0423m

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Packing:

40ug

Protein for Mouse Transcription factor SOX-2

Cat.:

R0423h

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Packing:

1mg

Purity:

>90%

Protein for Mouse Transcription factor SOX-2

Protein for Mouse Transcription factor SOX-2

R&D Technical Data

s

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

s

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

Recovery was determined by spiking various levels of SOX2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-104
Plasma	98	94-107

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SOX2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-103%	92-102%	89-106%	93-100%
EDTA plasma(n=5)	92-104%	90-107%	95-101%	94-104%
heparin plasma(n=5)	88-107%	94-103%	93-102%	91-106%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3."
Yuan H. , Corbi N. , Basilico C. , Dailey L.
Genes Dev.9:2635-2645(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

Yuan H. , Corbi N. , Basilico C. , Dailey L.
Submitted (1998-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION
3.

"A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2."
Collignon J. , Sockanathan S. , Hacker A. , Cohen-Tannoudji M. , Norris D. , Rastan S. , Stevanovic M. , Goodfellow P.N. , Lovell-Badge R.
Development122:509-520(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: 129.
4.

"Inhibition of DNA binding of Sox2 by the SUMO conjugation."
Tsuruzoe S. , Ishihara K. , Uchimura Y. , Watanabe S. , Sekita Y. , Aoto T. , Saitoh H. , Yuasa Y. , Niwa H. , Kawasuji M. , Baba H. , Nakao M.
Biochem. Biophys. Res. Commun.351:920-926(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION AT LYS-247;MUTAGENESIS OF LYS-247;SUBCELLULAR LOCATION;FUNCTION
5.

"Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors."
Takahashi K. , Yamanaka S.
Cell126:663-676(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: BIOTECHNOLOGY
6.

"SOX3 activity during pharyngeal segmentation is required for craniofacial morphogenesis."
Rizzoti K. , Lovell-Badge R.
Development134:3437-3448(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SOX3 AND FGFR1
7.

"Zfp206, Oct4, and Sox2 are integrated components of a transcriptional regulatory network in embryonic stem cells."
Yu H.B. , Kunarso G. , Hong F.H. , Stanton L.W.
J. Biol. Chem.284:31327-31335(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ZSCAN10
8.

"Ars2 maintains neural stem-cell identity through direct transcriptional activation of Sox2."
Andreu-Agullo C. , Maurin T. , Thompson C.B. , Lai E.C.
Nature481:195-198(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INDUCTION

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